Bradykinin induces a vapid cyclooxygenase-2 mRNA expression via Ca2+ mobilization in human gingival fibroblasts primed with interleukin-1 beta

We have previously demonstrated that bradykinin potentiates prostaglandin E , release in human gingival fibroblasts pretreated with interleukin-1 beta (priming), In this study, we demonstrate a potentiating effect of bradykini n on cyclooxygenase-2 mRNA expression in the interleukin-1 beta -primed fib roblasts. Interleukin-1 beta (200pg/ml) induced cyclooxygenase-2 mRNA expre ssion, but not bradykinin (1 CIM). However, bradykinin rapidly and markedly increased the cyclooxygenase-2 mRNA expression in the fibroblasts primed w ith interleukin-1 beta. In the primed fibroblasts, ionomycin and thapsigarg in mimicked the potentiating effect of bradykinin on the cyclooxygenase-2 m RNA expression. Dexamethasone and actinomycin D completely suppressed not o nly the interleukin-1 beta -induced cyclooxygenase-2 mRNA expression, but a lso the bradykinin-induced cyclooxygenase-2 mRNA expression in the interleu kin-1 beta -primed fibroblasts, although cycloheximide did not inhibit the effects of interleukin-1 beta and bradykinin. These results suggest that br adykinin-induced prostaglandin E, synthesis is regulated at the level of th e transcription of cyclooxygenase-2 mRNA via Ca2+ mobilization in the inter leukin-1 beta -primed human gingival fibroblasts. (C) 2001 Harcourt Publish ers Ltd.