Spectral and kinetic fluorescence properties of native and nonisomerizing retinal in bacteriorhodopsin

Authors
Haacke, S Vinzani, S Schenkl, S Chergui, M
Citation
S. Haacke et al., Spectral and kinetic fluorescence properties of native and nonisomerizing retinal in bacteriorhodopsin, CHEMPHYSCHE, 2(5), 2001, pp. 310-315
Citations number
34
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
CHEMPHYSCHEM
ISSN journal
14394235 → ACNP
Volume
2
Issue
5
Year of publication
2001
Pages
310 - 315
Database
ISI
SICI code
1439-4235(20010518)2:5<310:SAKFPO>2.0.ZU;2-H
Abstract
Wild-type bacteriorhodopsin (bR) and of bR reconstituted with a retinal ana log (bR5.12) were investigated with respect to their fluorescence propertie s. The latter is locked in an all-trans configuration due to the five-membe red ring spanning the C12-C13=C14 bonds (see structures). The large similar ity found in the spectra confirms very clearly that I-460, the fluorescent state of bR, is all-trans and that structural changes faster than torsion a re responsible for its formation.