IgH, TCR-gamma, and TCR-beta gene rearrangement in 80 B- and T-cell non-Hodgkin's lymphoma: Study of the association between proliferation and the so-called "aberrant" patterns
Mj. Garcia et al., IgH, TCR-gamma, and TCR-beta gene rearrangement in 80 B- and T-cell non-Hodgkin's lymphoma: Study of the association between proliferation and the so-called "aberrant" patterns, DIAGN MOL P, 10(2), 2001, pp. 69-77
Citations number
27
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
The current study analyzes the rearrangement pattern of immunoglobulin H (I
gH), T-cell receptor (TCR)-gamma, and TCR-beta genes in a group of 80 non-H
odgkin's lymphomas (NHL) of different histologic subtypes (43 B-cell and 37
T-cell types). The sensitivity and specificity provided by polymerase chai
n reaction amplification of these loci are evaluated. The association betwe
en the proliferation index and the presence of the so-called "aberrant" or
"dual" rearrangements is also considered. Ninety-one percent of B-celI NHL
showed IgH gene monoclonality, and 21% also exhibited a monoclonal pattern
in one of the TCR genes. Among T-cell NHL, the sensitivity of the study was
65% for the TCR-gamma gene and 46% for the TCR-P gene. The total sensitivi
ty was 76%, amplifying both loci. IgH gene aberrant rearrangements were obs
erved in 16% of T-cell neoplasms. A substantial percentage of dual rearrang
ements were detected in precursor and mature B- and T-cell NHL. B-cell NHL
showed a tendency toward higher values of proliferation when aberrant rearr
angements were present; however, this trend was not significant. Furthermor
e, in the case of T-cell NHL there was a significant negative association b
etween these two variables.