HMGN4, a newly discovered nucleosome-binding protein encoded by an intronless gene

Citation
Y. Birger et al., HMGN4, a newly discovered nucleosome-binding protein encoded by an intronless gene, DNA CELL B, 20(5), 2001, pp. 257-264
Citations number
35
Categorie Soggetti
Molecular Biology & Genetics
Journal title
DNA AND CELL BIOLOGY
ISSN journal
10445498 → ACNP
Volume
20
Issue
5
Year of publication
2001
Pages
257 - 264
Database
ISI
SICI code
1044-5498(200105)20:5<257:HANDNP>2.0.ZU;2-S
Abstract
We describe a newly discovered nuclear protein, HMGN4, that is closely rela ted to the canonical HMGN2 nucleosome-binding protein. The protein is encod ed by an intronless gene, which, in humans, is located in the hereditary he machromatosis region at position 6p21.3, A single similar to2-kb HMGN4 mRNA was found to be expressed, in variable amounts, in all human tissues teste d; however, the HMGN4 transcript was significantly less abundant than that of HMGN2, The HMGN4 protein could be detected in HeLa cells by Western anal ysis with an antibody elicited against a unique region of the protein. Tran sfection of HeLa cells with a plasmid expressing HMGN4-GFP indicated that t he protein localizes to the nucleus. Our results expand the multiplicity of the HMGN protein family and increase the known cellular repertoire of nucl eosome-binding proteins.