Ig. Casella et M. Gatta, Electrocatalysis and detection of carbohydrates by anion-exchange chromatography at a gold substrate electrode modified with nickel cyanide ions, ELECTROANAL, 13(7), 2001, pp. 549-554
Chemically modified electrodes obtained by direct interaction between gold
substrate and tetracyanonickelate(II) ions in alkaline medium were characte
rized by cyclic voltammetry toward the electrooxidation of carbohydrates. S
atisfactory performance with respect to catalytic stability, background cur
rent stability and lowering of detection limit was obtained for the analysi
s of ten oligosaccharides contained in a synthetic sample by anion-exchange
chromatography in alkaline solutions. Under constant applied potential of
0.55V (vs. Ag/AgCl), the detection limits (S/N=3) for all investigated comp
ounds ranged between 3 and 6 pmol injected for melezitose and turanose, res
pectively. The dynamic linear ranges spanned generally three orders of magn
itude above the limit of detection. The effects of the concentration of tet
racyanonickelate(II) ion on the catalytic activity, amperometric stability
and chromatographic separations were examined.