A non-invasive measure of changes in blood flow in the human anterior tibial muscle

We used photoplethysmography (PPG) to monitor blood flow changes in the hum an anterior tibial muscle during arterial occlusion and during isometric an d concentric contractions. Single-fibre laser-Doppler flowmetry (LDF) was u sed as a reference in 12 healthy subjects (5 men, 7 women; mean age 24 year s). Postexercise hyperaemic muscle blood flow (MBF) was measured immediatel y after isometric dorsiflexion of the ankle joint at maximal contraction fo r 1 min and full range-of-motion dorsiflexion and plantar flexion of the an kle joint for 1 min. A thigh tourniquet was applied for the evaluation of p ost-occlusive reactive hyperaemia. The MBF (baseline = 100%) was [mean (SD) ] 150 (31) % (P = 0.003) by PPG (880 nm) and 182 (66) % (P = 0.012) by LDF. After 1 min of maximal isometric contraction, MBF increased to 150 (51) % (P = 0.003) by PPG (880 nm) and to 169 (43) % (P = 0.005) by LDF. After 1 m in of maximal concentric contractions, MBF increased to 158 (59) % (P = 0.0 03) by PPG (880 nm) and to 170 (99) % (P = 0.008) by LDF. Skin blood flow, PPG (560 nm), did not change significantly after isometric or concentric co ntractions. The results indicate that reactive hyperaemia after exercise an d arterial occlusion can be assessed in the human anterior tibial muscle us ing PPG.