J. Schaap et Jh. Meijer, Opposing effects of behavioural activity and light on neurons of the suprachiasmatic nucleus, EUR J NEURO, 13(10), 2001, pp. 1955-1962
The mammalian circadian pacemaker is located in the suprachiasmatic nuclei.
It can be shifted in phase by photic cues and by the behavioural activity
of the animal. When presented together, light and behavioural activity atte
nuate each others' phase-shifting effect. Still unclear is how behavioural
activity affects the suprachiasmatic nuclei and how it interacts with photi
c information. Previously, we reported the occurrence of behaviourally indu
ced suppressions of neuronal activity. The present study investigates the c
haracteristics of these suppressions as a function of circadian time and, a
dditionally, in the presence of photic cues. We performed long-term multiun
it activity recordings of neurons in freely moving rats and found that thes
e suppressions of neuronal firing in the suprachiasmatic nucleus occurred a
t every phase of the circadian cycle. The magnitude of the suppressions sho
wed a circadian variation, with larger suppressions during subjective day.
When a light pulse was applied during a suppression, light and activity app
eared to oppose each others' effects within the recorded population of neur
ons. The resulting discharge level appeared to be the sum of both responses
. The opposing effects of light and activity were also found in single unit
recordings, indicating that photic and behavioural stimuli interact at the
level of a single neuron.