To assess the risk of contracting a waterborne disease from mineral water a
nd to implement control systems in the mineral water industry, knowledge of
the factors affecting the survival and multiplication of non-indigenous ba
cteria is needed. The survival of Escherichia coli, Salmonella typhimurium,
Staphylococcus aureus and Yersinia enterocolitica inoculated into a Portug
uese bottled mineral water was investigated under different conditions: lig
ht and dark, different recovery media, presence or absence of autochthonous
flora and different ages of water, based on their culturability. For all t
he pathogenic bacteria higher survival was obtained under dark conditions o
f storage and when enumeration was performed on non-selective media. Surviv
al of E. coli, Y. enterocolitica and S. typhimurium was longer in fresh wat
er than in old water; results obtained with S. aureus were not conclusive.
Survival of E. coli, Y. enterocolitica and S. typhimurium was lower in non-
sterile mineral water while the survival of S. aureus was lower in steriliz
ed mineral water. (C) 2001 Elsevier Science Ltd. All rights reserved.