Yw. Lin et al., Deletion mapping of chromosome 16q24 in hepatocellular carcinoma in Taiwanand mutational analysis of the 17-beta-HSD gene localized to the region, INT J CANC, 93(1), 2001, pp. 74-79
Human chromosome band 16q24 commonly undergoes loss of heterozygosity (LOH)
in human hepatocellular carcinoma (HCC). To further localize the region of
deletion on 16q24 and to evaluate the genetic role of 17-beto-HSD, which i
s near 16q24, in HCC, we examined the pattern of loss of heterozygosity in
88 HCC patients, DNAs from 88 pairs of HCCs and corresponding non-tumor par
ts were prepared. Loss of heterozygosity on chromosomes 16q24 was investiga
ted by 11 sets of microsatellite markers. Mutation analysis of type II 17-b
eta-HSD was performed by automatic sequencing, LOH on 16q24 for at least I
locus was found in 43 of the 88 tumor DNAs (49%), Three non-overlapping reg
ions of frequent LOH were defined in these 43 tumors with partial deletions
. The first region was between D16S516 loci and D16S507, encompassed by a I
-cM region, defined by the D16S504, The second region was defined by the 17
HSDB2 locus between D16S505 and D16S422, encompassed approximately by a I-c
M region. The third region was between D16S520 and D16S413, defined by D16S
3048, encompassed approximately by a 4-cM region, Homozygous deletions of a
ny exons in 17HSDB2 gene were identified in 7 of 27 cases (26%), Automated
sequencing analysis of 17HSDB2 failed to demonstrate mutations in any of th
ese specimens, Our data suggest that the 17HSDB2 locus is a frequent target
of deletion in HCC but the inactivation of 17H5DB2 may not involve sequenc
e mutations, Furthermore, the presence of the other 2 frequent LOH regions
suggest that the putative tumor suppressor genes at these locations might b
e involved in the development of HCC, (C) 2001 Wiley-Liss, Inc.