Proposal of Mycetocola gen. nov in the family Microbacteriaceae and three new species, Mycetocola saprophilus sp nov., Mycetocola tolaasinivorans sp nov and Mycetocola lacteus sp nov., isolated from cultivated mushroom, Pleurotus ostreatus

The taxonomic positions of 10 tolaasin-detoxifying bacteria, which were iso lated from the cultivated mushroom Pleurotus ostreatus, were investigated. These strains are Gram-positive, obligately aerobic, non-sporulating and ir regular rod-shaped bacteria. They have the following characteristics: the m ajor menaquinone is MK-10, the DNA G+C content ranges from 64 to 65 mol%, t he diamino acid in the cell wall is lysine and the muramic acid in the pept idoglycan is an acetyl type. The major fatty acids are anteiso-C-15:0 and a nteiso-C-17:0. On the basis of morphological, physiological and chemotaxono mic characteristics, together with DNA-DNA reassociation values and 16S rRN A gene sequence comparison data, the new genus Mycetocola gen, nov. is prop osed for these bacteria in the family Microbacteriaceae and three new speci es are also proposed: Mycetocola saprophilus sp. nov. (type strain CM-01(T) = IFO 16274(T) = MAFF 211324(T) = NRRL B-24119(T)), Mycetocola tolaasinivo rans sp, nov. (type strain CM-05(T) = IFO 16277(T) = MAFF 211325(T) = NRRL B-24120(T)) and Mycetocola lacteus sp, nov. (type strain CM-10(T) = IFO 162 78(T) = MAFF 211326(T) = NRRL B-24121(T)). The type species of the genus is Mycefocola saprophilus sp, nov.