Seven strains of an unknown Gram-positive catalase-negative chain-forming c
occus-shaped organism isolated from clinical specimens from sheep were char
acterized by phenotypic and molecular taxonomic methods. Comparative 16S rR
NA gene sequencing studies demonstrated that the bacterium represents a new
sub-line within the genus Streptococcus, The unknown bacterium was readily
distinguished from recognized streptococcal species by biochemical tests a
nd electrophoretic analysis of whole-cell proteins. Based on phylogenetic a
nd phenotypic evidence, it is proposed that the unknown bacterium be classi
fied as Streptococcus ovis sp, nov. The type strain of Streptococcus ovis i
s CCUG 39485(T) (= LMG 19174(T)).