Burkholderia fungorum sp nov, and Burkholderia caledonica sp nov., two newspecies isolated from the environment, animals and human clinical samples

A polyphasic taxonomic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analyses, whole-cell protein and f atty acid analyses and an extensive biochemical characterization was perfor med on 16 strains isolated from the environment, animals and human clinical samples. The isolates belonged to the genus Burkholderia, were phylogeneti cally closely related to Burkholderia graminis, Burkholderia caribensis and Burkholderia phenazinium and had G+C contents between 61.9 and 62.2 mol%. Seven strains isolated from the rhizosphere were assigned to Burkholderia c aledonica sp. nov. [type strain LMC 19076(T) (= CCUG 422363(T))]. Nine stra ins isolated from the environment, animals and human clinical samples were assigned to Burkholderia fungorum sp. nov. [type strain LMG 16225(T) (= CCU C 31961(T))]. Differential tests for B. graminis, B. caribensis, B. phenazi nium, B. caledonica and B. fungorum include the following: assimilation of trehalose, citrate, DL-norleucine, adipate and sucrose; nitrate reduction; growth in the presence of 0.5 % NaCl; and beta -galactosidase activity.