Z. Lei et al., Use of methanolysis for the determination of total ellagic and gallic acidcontents of wood and food products, J AGR FOOD, 49(3), 2001, pp. 1165-1168
Anhydrous methanolic HCl has been found to be an excellent reagent for rele
asing ellagic acid and gallic acid (as methyl gallate) from biomass substra
tes. Optimization of both the reaction conditions and the gradient HPLC ana
lysis has led to the development of a new protocol. The method provides ell
agic acid yields significantly higher than those obtained previously, indic
ating total ellagic acid contents of several substrates have previously bee
n underestimated.