Simultaneous determination of thiamin and riboflavin in mushrooms by liquid chromatography

A simple, fast, inexpensive, and reliable method useful for the simultaneou s;routine determination of thiamin and riboflavin in mushrooms is examined, it uses the extraction procedure, with slight modifications, proposed by t he AOAC for the extraction of thiamin and riboflavin, followed by a liquid chromatographic separation on a reversed-phase Spherisorb ODS column with m ethanol/ water as mobile phase gradient. Fluorometric detection is used at the following excitation and emission wavelengths, respectively, 360 and 42 5 nm in the case of thiamin and 422 and 515 nm for riboflavin. The analytic al parameters of linearity, the precision of the method (RSD = 2.45 and 2.5 1% for thiamin and riboflavin, respectively), and the results of the compar ison with the corresponding AOAC fluorometric methods show that the studied method is useful for the measurement of thiamin and riboflavin in fresh mu shrooms.