Genetic variations of randomly amplified polymorphic DNA polymorphisms in Taoyuan and Duroc pigs

The purposes of this study were to assess the genetic v between Taoyuan (T) and Duroc (D) pigs using randomly amplified polymorphic DNA (RAPD) fingerp rints and to evaluate the genetic relationship to a commercial synthetic li ne-Taiwan Black (TB) pig (75% D, 25% T). To assess the genetic variability between T and D, 71 random primers (Operon) were used for RAPD fingerprinti ng by polymerase chain reaction (PCR). The evaluation of the genetic relati onship was based on band sharing frequency and band frequency. Thirty-five of the 71 primers polymorphisms in RAPD fingerprints of T or D pigs. Twenty -two primers produced polymorphic bands from only T genomic DNA, and 14 pri mers could produce polymorphic bands from only D genomic DNA. These results indicated that there was some generic difference between T and D pigs. The within-population genetic similarity (WGS) for T, D, and TB populations we re 0.742, 0.747;and 0.745, respectively, the between-population genetic sim ilarity (BGS) was 0.946 between T and TB; 0.953 between D and TB; and 0.934 between D and T. The parameters of genetic distance between T and TB; D an d TB, T and D were 0.080, 0.064, and 0.096, respectively. When the values o f genetic similarity and genetic distance between populations estimated as frequency of occurrence of bands showed lower genetic similarities between pig populations, but indicted similar relationship. TB was genetically more related to D than to T. It provided evidence of the usefulness of the RAPD technique to determine genetic relatedness among T, D, and TB.