We describe the utilization of a red fluorescent protein (DsRed) as an in v
ivo marker for Saccharomyces cerevisiae. Clones expressing red and/or green
fluorescent proteins with both cytoplasmic and nuclear localization were o
btained. A series of vectors are now available which can be used to create
amino-terminal (N-terminal) and carboxyl-terminal (C-terminal) fusions with
the DsRed protein.