Synergistic chiral separations using the glycopeptides ristocetin A and vancomycin

The effectiveness of using a mixture of the chiral selectors vancomycin and ristocetin A to achieve chiral recognition was examined in this study. The results of using the mixed chiral selector vancomycin and ristocetin A in capillary electrophoresis were compared with the results of using each chir al selector alone. Chiral separations were carried out using a coated capil lary column to suppress electroosmotic flow and minimize interactions with the capillary wall. We employed a countercurrent process where the solute r eaches the detection cell window after the chiral selector has cleared the window, minimizing the background absorbance from the chiral selector and i mproving sensitivity. Using a mixture of vancomycin and ristocetin A, separ ations were achieved which often exceeded the resolving power of tither chi ral selector when used alone. The effect of voltage on resolution was also studied, and the optimal voltage was found to be between -5 and - 8 kV. (C) 2001 Elsevier Science B.V. All rights reserved.