Modification of peroxidase with telomers containing amino end-group and their catalytic activity

Two telomers containing amino end-group are synthesized and attached covale ntly to peroxidase by using coupling agents. When the enzyme is modified wi th a telomer having polystyrene as a main chain, it dissolves in organic so lvents and exhibits catalytic activity in water-immiscible solvent. The cat alytic activity increases as the polarity of the solvents decreases. The en zyme modified with a telomer having poly- N-isopropylacrylamide chain varie s its solubility drastically from water-soluble to water insoluble at a cer tain temperature, being accompanied by change in the catalytic activity. Th e solubility variation is reversible against temperature change and allows the catalytic activity to be controlled reversibly.