Determination of etoposide in human plasma and leukemic cells by high-performance liquid chromatography with electrochemical detection

This paper describes a high-performance liquid chromatographic method with electrochemical detection for the determination of etoposide levels in plas ma, total and non-protein bound concentration, and in leukemic cells. The p recision for between-runs (n=6) was 7.0, 4.9, and 9.5%, the accuracy was 3. 7, 7.1 and 6.3%, and within-runs precision (n=6) was 3.9, 2.9 and 5.1% for total plasma, non-protein bound plasma fraction and leukemic cells, respect ively. The correlation coefficients (R-2) were 1.00 for all calibration cur ves. These assays have been applied to analyze samples from one patient wit h acute myelogenous leukemia during 24 h after i.v. infusion of etoposide ( 100 mg/m(2)). (C) 2001 Elsevier Science B.V. All rights reserved.