Optimization of electrophoresis for the identification of low molecular mass allergens in hazelnuts

Authors
Schocker, F Becker, WM
Citation
F. Schocker et Wm. Becker, Optimization of electrophoresis for the identification of low molecular mass allergens in hazelnuts, J CHROMAT B, 756(1-2), 2001, pp. 105-111
Citations number
22
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
13872273 → ACNP
Volume
756
Issue
1-2
Year of publication
2001
Pages
105 - 111
Database
ISI
SICI code
1387-2273(20010525)756:1-2<105:OOEFTI>2.0.ZU;2-Z
Abstract
Conventional electrophoresis techniques used to identify food allergens are insufficient to separate low molecular mass proteins and peptides. In this paper we performed three different methods which provided an extended reso lving power for small proteins. Applying the improved techniques, we were a ble to separate hazelnut proteins into distinct bands below 10 kDa. (C) 200 1 Elsevier Science B.V. All rights reserved.