Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity

Grid-immunoblotting is a procedure that allows the simultaneous testing of up to 20 different antibodies such as monoclonal antibody-containing hybrid oma supernatants or human sera for specific antibodies to up to 20 differen t antigens or allergens on a single sheet of nitrocellulose membrane. Since only 150 to 200 mul of antibody-containing solution are required this tech nique is uniquely suited to test growing hybridomas and small amounts of se ra (e.g. mouse and children's sera). Compared to a standard ELISA, approxim ately ten times less antibody is needed to obtain the same information. (C) 2001 Published by Elsevier Science B.V.