Alkaline phosphatase from Jawala shrimp (Acetes indicus)

Authors
Chander, R Thomas, P
Citation
R. Chander et P. Thomas, Alkaline phosphatase from Jawala shrimp (Acetes indicus), J FOOD BIOC, 25(2), 2001, pp. 91-103
Citations number
17
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF FOOD BIOCHEMISTRY
ISSN journal
01458884 → ACNP
Volume
25
Issue
2
Year of publication
2001
Pages
91 - 103
Database
ISI
SICI code
0145-8884(200105)25:2<91:APFJS(>2.0.ZU;2-7
Abstract
Alkaline phosphatase M,as isolated from Jawala shrimps (Acetes indicus), a tiny crustacean. The hepatopancreas (head region) is a good source of this enzyme. pH optima of the partial purified enzyme was found to be 9.5 and op timal temperature for maximal activity was 40C. Jawala phosphatase was comp letely inhibited by 1, 10-phenanthroline and EDTA, indicating that it is a metalloprotein. The activity, of inhibited enzyme was restored by Zn2+ and Mn2+ salts. Feed back inhibition of the enzyme by inorganic phosphate was a lso observed.