Alkaloid composition of Lupinus campestris from Mexico

The content of quinolizidine alkaloids (QA) in Lupinus campestris, Fabaceae family: was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Samp les of various organs of Lupinus campestris collected at different monthly, stages of the growing plant, were subjected to extraction in a Merck Extre lut column. The quinolizidine alkaloid patterns of stems, leaves, flowers, pods and seen's were assessed and then identified and quantified by GC. Alk aloid structures were identified according to their mass fragmentation patt erns, in combination with their indicative Kovats retention inner. Alkaloid s found in several developmental stages of the plant were mainly: aphyllidi ne, 5,6-dehydrolupanine, aphylline, dehydro-oxosparteine lupanine, alpha -i solupanine, hydroxyaphylline and hydroxyaphyllidine, plus two alkaloids tha t were not identified During the third month the relative abundance of tota l alkaloids were highest. The main alkaloids found in seeds were hydroxyaph ylline and hydroxyaphllidine.