A considerable amount of squid mantle collagen, 45% to 70% of total collage
n, was not solubilized even after 30 min of heating in boiling water with i
ts fibrous structure left intact. Pyridinoline, one of the major intermolec
ular crosslinks in matured collagen, was more predominantly included in the
insoluble collagen than in the soluble one (p < 0.05). These results sugge
st that pyridinoline is closely related to the heat solubility of squid col
lagen.