Epstein-Barr virus-encoded protein kinase BGLF4 mediates hyperphosphorylation of cellular elongation factor 1 delta (EF-1 delta): EF-1 delta is universally modified by conserved protein kinases of herpesviruses in mammalian cells

Translation elongation factor 1 delta (EF-1 delta) is hyperphosphorylated i n various mammalian cells infected with alpha-, beta- and gammaherpesviruse s and EF-1 delta modification is mediated by viral protein kinases, includi ng UL13 of herpes simplex virus type 1 and UL97 of human cytomegalovirus. I n this study, the following is reported, (i) BGLF4 encoded by the prototype gammaherpesvirus Epstein-Barr virus was purified as a fusion protein that was labelled with [gamma-P-32]ATP and labelling was eliminated by phosphata se. (ii) The ratio of the hyperphosphorylated form of human EF-1 delta was increased both in Sf9 cells after infection with baculoviruses expressing G ST-BGLF4 fusion proteins and in COS-7 cells after transfection with a BGLF4 expression plasmid, These results indicate that purified BGLF4 possesses p rotein kinase activity and mediates EF-1 delta hyperphosphorylation. These data also support the hypothesis that the protein kinases that are conserve d by herpesviruses universally mediate EF-1 delta modification in mammalian cells.