Equipotent generation of protective antitumor immunity by various methods of dendritic cell loading with whole cell tumor antigens

Multiple clinically applicable methods have been used to induce dendritic c ells (DCs) to express whole cell tumor antigens, including pulsing DCs with tumor lysate, and mixing DCs with apoptotic or live tumor cells. Herein we demonstrate, using two different tumor systems, that these methods are equ ipotent inducers of systemic antitumor immunity. Furthermore, tumor lysate pulsed DC vaccines generate more potent antitumor immunity than immunizatio n with irradiated tumor cells plus the classic adjuvant, Corynebacterium pa rvum.