Intestinal absorption of specific structured triacylglycerols

To clarify the intestinal absorption pathway of medium-chain fatty acids fr om MMM-type structured triaclyglycerols containing both medium- and long-ch ain fatty acids, we studied the lymphatic transport of 1,3-dioctanoyl-2-lin oleoyl-sn-glycerol (8:0/18:2/8:0), 1,3-didecanoyl-2-linoleoyl-sn-glycerol ( 10:0/18:2/10:0), and 1,3-didodecanoyl-2-linoleoyl-sn-glycerol (12:0/18:2/12 :0) in a rat model. Safflower oil was used in the absorption study in order to compare the absorption of medium-chain fatty acids and long-chain fatty acids, The triacylglycerol species of lymph Lipids were separated on a rev ersed-phase high performance Liquid chromatograph (RP-HPLC) and identified by atmospheric pressure chemical ionization mass spectrometry, The composit ion of triacylglycerols was quantified by RP-HPLC with evaporative Light sc attering detection. The intact MLM-type triacylglycerols were detected in t he lymph lipids after administration of the specific structured triacylglyc erols (STAG), The recoveries of 8:0/18:2/8:0, 10:0/18:2/10:0, and 12:0/18:2 /12:0 were 0.6%, 12%, and 5%, respectively, Several new triacylglycerol spe cies were detected in the lymph Lipids, including MLL-, LLL-, and MMM-type triacylglycerols. From the present study we conclude that the medium-chain fatty acids from STAG, in addition to absorption into the portal blood as f ree fatty acids, are absorbed by the same pathway as the conventional long- chain triacylglycerols, that is, they are hydrolyzed into free fatty acids, absorbed and activated into CoA, and reacylated into triacylglycerols in t he enterocyte, The hydrolysis of MLM-type STAG is predominantly partial hyd rolysis, whereas part of the STAG can also be hydrolyzed to free glycerol a nd free fatty acids. - Mu, H., and CE. Hoy. Intestinal absorption of specif ic structured triacylglycerols.