Persistence of a clone of methicillin-resistant Staphylococcus aureus in aburns unit

A total of 128 MRSA isolates from a burns unit in 1992 and 1997 was studied by resistotyping, plasmid analysis and pulsed-field gel electrophoresis (P FGE) of SmaI-digested chromosomal DNA to ascertain whether a clone of MRSA had persisted in the unit or whether different clones had been introduced a t different times. All the MRSA isolates produced B-lactamase and had high MICs to methicillin (> 256 mg/L), All were resistant to tetracycline, kanam ycin, cadmium acetate and mercuric chloride. Most were resistant to gentami cin, neomycin, erythromycin, chloramphenicol, trimethoprim, ciprofloxacin, propamidine isethionate and ethidium bromide, and were susceptible to minoc ycline, vancomycin and teicoplanin, None of the 1992 isolates was resistant to mupirocin, but 56% and 19% of the 1997 isolates expressed high- and low -level mupirocin resistance, respectively. Many of the 1997 isolates had ac quired a 38-kb plasmid encoding high-level mupirocin resistance. The 1992 i solates had two main PFGE patterns; 82% of them belonged to PBGE pattern 1, The 1997 isolates had PFGE pattern 1, the same as the majority of the 1992 isolates. All MRSA isolates from both years carried the mecA gene in the s ame SmaI fragment. These findings demonstrated that a clone of MRSA that wa s prevalent in the burns unit in 1992 had persisted and became the predomin ant clone in 1997.