A modified rapid method of nucleic acid isolation from suspension of matured virus: applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate

This report describes a method for the isolation of nucleic acid from a sus pension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. In stead of the usual method of ultracentifugation, a filtration method was ap plied to concentrate the virus rapidly and nucleic acid was then isolated b y a standard phenol/chloroform/isoamyl-alcohol extraction procedure. The DN A was found to be sufficiently purified to generate a reproducible restrict ion endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIII when comp ared with the prototype strain.