Brachycerine (1), an unusual alkaloid from the leaves of Psychotria brachyc
eras, was characterized through spectroscopic data interpretation and its s
tereochemistry established by NOE difference techniques. Brachycerine (1) w
as found to be restricted to shoots in rooted cuttings of P. brachyceras (0
.018 +/- 0.094% dry weight), and accumulation was unaffected by root induct
ion treatment with auxin.