Physicochemical evaluation of nanoparticles assembled from poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) block copolymers as drug delivery vehicles

Nanoparticles assembled from poly(D,L-lactic acid)-poly(ethylene glycol) (P LA-PEG) block copolymers may have a therapeutic application in site-specifi c drug delivery. A series of AB block copolymers based on a fixed PEG block (5 kDa) and a varying PLA segment (2-110 kDa) have been synthesized by the ring-opening polymerization of D,L-lactide using stannous octoate as a cat alyst. These copolymers assembled to form spherical nanoparticles in aqueou s media following precipitation from a water-miscible organic solvent. H-1 NMR studies of the PLA-PEG nanoparticles in D2O confirmed their core-shell structure, with negligible penetration of the hydrated PEG chains into the PLA core. The influence of the PLA block molecular weight on the hydrodynam ic size and micellar aggregation number of the assemblies was determined by dynamic and static light scattering techniques. The hydrodynamic radius of the PLA-PEG 2:5-30:5 nanoparticles was solely dependent on the copolymer a rchitecture and scaled linearly as N-PLA(1/3) where N-PLA is the number of monomeric units in the PLA block. The PEG chains of the small PLA-PEG 2:5 a nd 3:5 assemblies appeared to be fairly splayed as a consequence of their r elatively low aggregation number and high surface coverage. However, as NPL A was increased to 6 kDa the area available per PEG chain at the periphery of the shell decreased significantly and then remained fairly constant with further increases in the molecular weight of the PLA block. The aggregatio n number and hence particle size of nanoparticles produced from copolymers with a PLA block of 45 kDa or more was found to also depend on the concentr ation of copolymer dissolved in the organic phase during preparation. This suggested that that the PEG chains had little influence on the assembly of the higher molecular weight copolymers.