Differences in genetic changes between multiple myeloma and plasma cell leukemia demonstrated by comparative genomic hybridization

Citation
Nc. Gutierrez et al., Differences in genetic changes between multiple myeloma and plasma cell leukemia demonstrated by comparative genomic hybridization, LEUKEMIA, 15(5), 2001, pp. 840-845
Citations number
33
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
LEUKEMIA
ISSN journal
08876924 → ACNP
Volume
15
Issue
5
Year of publication
2001
Pages
840 - 845
Database
ISI
SICI code
0887-6924(200105)15:5<840:DIGCBM>2.0.ZU;2-M
Abstract
To analyze the genomic differences between multiple myeloma (MM) and plasma cell leukemia (PCL), a total of 30 cases were studied by comparative genom ic hybridization (CGH). In five cases with a low proportion of plasma cells (PC) in bone marrow, an enrichment of PC was performed by using immunomagn etic beads conjugated with the monoclonal antibody 8-84. In 24 out of the 2 5 MM (96%) and in all five PCL (100%) patients DNA copy number changes were identified by CGH analysis; in the NIM case without chromosomal imbalances , the immunomagnetic enrichment of PC had failed. The most recurrent change s in MM patients were gains at chromosomes 15q (48%), 11q (44%), 3q (40%), 9q (40%) and 1q (36%). By contrast, all PCL patients showed gains in Iq. Lo sses of chromosomal material were significantly more frequent in PCL than i n MM patients (P = 0,03): losses on 13q in 80% of PCL vs 28% of MM; and on chromosome 16 in 80% vs 12%, respectively. In addition, PCL patients showed losses of 2q and 60 that were not present in MM. The CGH data show differe nces in chromosomal imbalances between MM and PCL.