Single-voxel oversampled J-resolved spectroscopy of in vivo human prostatetissue

Single-voxel J-resolved spectroscopy with oversampling in the F, dimension was used to obtain water unsuppressed H-1 spectra of in situ human prostate tissue in 40 previously untreated prostate cancer patients. Based on T-2-w eighted MRI and previous biopsy information, voxels were placed in regions of benign or malignant peripheral zone tissue, or in regions of predominant ly glandular or stromal benign prostatic hyperplasia (BPH) within the centr al gland, The addition of a second J-resolved dimension allowed for the obs ervation of the J-modulation of citrate, as well as the resolution of polya mines from overlapping choline and creatine signals, Regions of healthy per ipheral zone tissue and glandular BPH all demonstrated high levels of citra te and polyamines, with consistent coupling and J-modulation patterns. Conv ersely, regions of malignant peripheral zone tissue and stromal BPH demonst rated low levels of citrate and polyamines consistent with prior in vivo an d ex vivo studies. Moreover, water T,relaxation times determined for health y peripheral zone tissue (mean 128 +/- 15.2 msec) were significantly differ ent than for malignant peripheral zone tissue (mean 88.0 +/- 14.2 msec, P = 0.005), as well as for predominantly glandular (mean 92.4 +/- 12.2 msec, P = 0.009) and stromal BPH (mean 70.9 +/- 12.1 msec, P = 0.003), This prelim inary study demonstrates that J-resolved spectroscopy of the in situ prosta te can be acquired, and the information obtained from the second spectral d imension can provide additional physiologic information from human prostate tissue in a reasonable amount of time (< 10 min). (C) 2001 Wiley-Liss, Inc .