Effect of low voltage electrical stimulation and temperature conditioning on postmortem changes in glycolysis and calpains activities of Korean native cattle (Hanwoo)

The combined effects of low voltage electrical stimulation (LVES) and tempe rature conditioning during early postmortem (PM) ageing on glycolytic rates and calpains activities of Korean native cattle (Hanwoo) were determined. M. longissimus was taken after splitting course, divided into three pieces and temperature conditioned at 2, 16, and 30 degreesC for 3 h PM. The PM gl ycolytic rates, calpains and calpastatin activities were measured at 1, 3, 9, and 24 h PM. Although both LVES and the 30 degreesC treatment accelerate d glycolytic rates and resulted in improved enzyme activities, LVES was mor e effective for the improvement of enzyme activities than the 30 degreesC t reatment. Among tested Rigor-values (R-value; R-248, R-250, and R-258), R-2 58 showed the highest correlation with pH (r = 0.814, P < 0.01), glycogen c ontent (r = 0.784, P < 0.01) and mu -calpain (r = 0.838, P < 0.01) and sele cted as a suitable parameter to predict glycolytic rate. The high correlati on coefficients between <mu>-calpain activity and metabolic rate parameters suggest that the change in the enzyme activity is closely related to glyco lytic rates. The LVES in combination with the 30 degreesC treatment until 3 h PM was the best treatment to accelerate the glycolytic rate and to impro ve the calpains activities in Hanwoo tissue. (C) 2001 Elsevier Science Ltd. All rights reserved.