Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation

Authors
Frohn, JT Knapp, HF Stemmer, A
Citation
Jt. Frohn et al., Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation, OPTICS LETT, 26(11), 2001, pp. 828-830
Citations number
9
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science","Optics & Acoustics
Journal title
OPTICS LETTERS
ISSN journal
01469592 → ACNP
Volume
26
Issue
11
Year of publication
2001
Pages
828 - 830
Database
ISI
SICI code
0146-9592(20010601)26:11<828:TREIFM>2.0.ZU;2-L
Abstract
A method for increasing lateral as well as axial resolution in fluorescence microscopy is presented. A pass-band with a high cutoff frequency througho ut reciprocal space can be achieved by illumination of the object with spat ially harmonic excitation patterns generated by the interference of two col limated laser beams. Theoretical calculations show an almost isotropic poin t-spread function with a FWHM near 100 nm. (C) 2001 Optical Society of Amer ica.