Lissencephaly is a severe brain malformation in humans. To study the functi
on of the gene mutated in lissencephaly (LIS1), we deleted the first coding
exon from the mouse Lis1 gene. The deletion resulted in a shorter protein
(sLIS1) that initiates from the second methionine, a unique situation becau
se most LIS1 mutations result in a null allele. This mutation mimics a muta
tion described in one lissencephaly patient with a milder phenotype. Homozy
gotes are early lethal, although heterozygotes are viable and fertile. Most
strikingly, the morphology of cortical neurons and radial glia is aberrant
in the developing cortex, and the neurons migrate more slowly. This is the
first demonstration, to our knowledge, of a cellular abnormality in the mi
grating neurons after Lis1 mutation. Moreover, cortical plate splitting and
thalomocortical innervation are also abnormal. Biochemically, the mutant p
rotein is not capable of dimerization, and enzymatic activity is elevated i
n the embryos, thus a demonstration of the in vivo role of LIS1 as a subuni
t of PAF-AH. This mutation allows us to determine a hierarchy of functions
that are sensitive to LIS1 dosage, thus promoting our understanding of the
role of LIS1 in the developing cortex.