Genetic diversity in Penaeus chinensis shrimp as revealed by RAPD technique

The random amplified polymorphic DNA analysis was used to estimate genetic diversity in one successively cultivated stack and three wild stocks of Pen aeus chinensis shrimp, two of which were collected from the spawning and wi ntering grounds in the west coast of Korean peninsula, and one from the fee ding ground in the China coast of the Yellow Sea. A random primer kit was e mployed to scan the genomic DNA in 20 individuals of each index stock. A to tal of 110 reproducible RAPD markers were obtained, 68.2% of which showed a sound conformability within all the individuals detected, implying that th e genetic variability in P. chinesis is relatively low. The proportions of polymorphic loci among these four stocks ranged from 20% to 33.3%, while th e degrees of genetic polymorphisms varied from 0.0093 to 0.0307. The geneti c variability of inter-stocks was higher than that of inc-a-stock. The gene tic diversity in different stocks differed from each other; that is, a less genetic differentiation in the spawning and wintering stocks from the west coast of Korean Peninsula was revealed and their genetic diversities were higher than that of the spawning stock in the Bohai Sea and the China coast of the Yellow Sea. As detected, the genetic diversity in the successively cultivated stock was the lowest among these four stocks. Through genetic di stance analysis between a random pair of individuals, a dendrogram of the a bove-mentioned four stocks was constructed by unweighted pair group method with arithmetic mean. The results based on cluster analysis well fitted wit h the geographical distribution of P. chinesis in the Bohai and Yellow Seas .