K. Li et Tp. West, EFFECT OF THE CARBON SOURCE SUCCINATE ON PYRIMIDINE SYNTHESIS IN BURKHOLDERIA-CEPACIA, Journal of basic microbiology, 37(3), 1997, pp. 213-216
The effect of the carbon source succinate on pyrimidine synthesis in t
he pathogen Burkholderia cepacia ATCC 25416 was investigated. The five
de novo pathway enzyme activities of pyrimidine biosynthesis in B. ce
pacia were shown to be affected when ATCC 25416 cells were grown in th
e presence of uracil. Pyrimidine limitation of a uracil auxotrophic B.
cepacia strain lacking orotidine 5'-monophosphate decarboxylase activ
ity increased aspartate transcarbamoylase, dihydroorotase, dihydroorot
ate dehydrogenase and orotate phosphoribosyltransferase activities by
at least 1.5-fold which indicated that these pathway enzymes may be re
gulated by a uracil-related compound. The levels of the de novo pyrimi
dine biosynthetic enzyme activities in B. cepacia ATCC 25416 cells app
eared to be influenced to a greater degree in cells grown on succinate
than on glucose as a carbon source.