I. Vucenik et al., Usefulness of galactose oxidase-Schiff test in rectal mucus for screening of colorectal malignancy, ANTICANC R, 21(2B), 2001, pp. 1247-1255
Based on a "field-effect" theory in colon carcinogenesis, and the expressio
n of the disaccharide tumor marker D galactose-beta-[1 -->3]-N-acetyl-D-gal
actosamine (Gal-GalNAc) in the rectal mucus of patients with cancer and pre
cancer of the colon, Shamsuddin developed a simple, accurate, inexpensive,
easy to perform and rapid (less than or equal to 15 min) screening test for
colonic cancer and precancerous lesions. In this study we examined 137 rec
tal mucus samples of randomly selected patients with cob ectal malignancy o
r other colorectal diseases to confirm the sensitivity and specificity of t
his test in Croatia. Additionally, to test the validity of the "field-effec
t" theory, that the mucosa away from the obvious cancer will show abnormali
ties as a result of the generalized effect of the carcinogen throughout the
entire field of the target tissue, we also monitored a subset of 53 patien
ts post-operatively. Individuals free of colonic or any other malignancies
served as control (n=31). The rectal mucin was smeared on membrane filter a
nd developed by a sequential reaction of galactose oxidase (GO) and Schiffs
reagent. The test results were correlated to the findings from colonoscopy
/surgery and histopathology. The sensitivity of the test was shown to be 10
0% and the specificity was 96.8% (p <0.001). Interestingly, the rest was po
sitive in 60% (32 of 53) bf the samples collected from patients after tumor
resection, showing the persistence of the biochemical changes even though
malignant tumors were removed, hence supporting the field-effect phenomenon
of carcinogenic stimuli. Five patients out of these 32 (16%) postoperative
cases with positive GO test had a tumor recurrence within a year (0.05 <p
<0.10), suggesting that a persistently positive GO test in this population
may serve as a predictor of tumor recurrence. We conclude that Gal-GalNAc i
s an early and intermediate biomarker, suitable not only for the detection
of malignancy in ifs inception, but also for monitoring of people at high r
isk for cancer; and the efficacy of the cancer therapy as well as secondary
prevention by this technology.