NONEXPONENTIAL KINETICS OF A SINGLE TRNA(PHE) MOLECULE UNDER PHYSIOLOGICAL CONDITIONS

Authors
JIA YW SYTNIK A LI LQ VLADIMIROV S COOPERMAN BS HOCHSTRASSER RM
Citation
Yw. Jia et al., NONEXPONENTIAL KINETICS OF A SINGLE TRNA(PHE) MOLECULE UNDER PHYSIOLOGICAL CONDITIONS, Proceedings of the National Academy of Sciences of the United Statesof America, 94(15), 1997, pp. 7932-7936
Citations number
30
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
94
Issue
15
Year of publication
1997
Pages
7932 - 7936
Database
ISI
SICI code
0027-8424(1997)94:15<7932:NKOAST>2.0.ZU;2-B
Abstract
The fluorescence decay functions of individual, specifically labeled t RNA(Phe) molecules exhibit nonexponential character as a result of con formational dynamics occurring during the measurement on a single mole cule. tRNA(Phe) conformational states that interchange much more slowl y are evidenced by the distribution of lifetimes observed for many ind ividual molecules. A structural model fur the nonexponential decay ind icates that the tRNA(Phe)-probe adduct fluctuates between two states, one of which provides conditions that quench the probe fluorescence.