Jr. Boelaert et al., The additive in vitro anti-HIV-1 effect of chloroquine, when combined withzidovudine and hydroxyurea, BIOCH PHARM, 61(12), 2001, pp. 1531-1535
The 4-aminoquinoline chloroquine and its analogue hydroxychloroquine are en
dowed with anti-HN-l activity both in vitro and in vivo. We previously repo
rted that the addition of CQ (chloroquine) to the combination of HU (hydrox
yurea) and ddI (didanosine) provides additive anti-HIV-l activity. We here
extended this in vitro investigation by studying whether the addition of CQ
also resulted in additive anti-HIV-1 activity when combined with HU plus A
ZT (zidovudine). The same effect was found, whether CQ was added to HU plus
AZT or to HU plus ddI, in recently infected H-9 and U-937 cells or primary
T cells and monocytes, as well as in immunologically or oxidatively stimul
ated ACH-2 and U-l tells. At concentrations where CQ exerts its anti-HIV-l
effect in combination with the other drugs, CQ addition does not result in
either cell toxicity or apoptosis. (C) 2001 Elsevier Science Inc. All right
s reserved.