B. Nal et al., Location analysis of DNA-bound proteins at the whole-genome level: untangling transcriptional regulatory networks, BIOESSAYS, 23(6), 2001, pp. 473-476
In this post-sequencing era, geneticists can focus on functional genomics a
n a much larger scale than ever before. One goal is the discovery and eluci
dation of the intricate genetic networks that co-ordinate transcriptional a
ctivation in different regulatory circuitries. High-throughput gene express
ion measurement using DNA arrays has thus become routine strategy. This app
roach, however, does not directly identify gene loci that belong to the sam
e regulatory group; e,g,, those that are bound by a common (set of) transcr
iption factor(s), Working in yeast, two groups have recently published an e
legant method that could circumvent this problem, by combining chromatin im
munoprecipitation and DNA micro-arrays.((1,2)) The method is likely to prov
ide a powerful arrays. tool for the dissection of global regulatory network
s in eukaryotic cells. (C) 2001 John Wiley & Sons, Inc.