Enantioselective high-performance liquid chromatography of therapeuticallyrelevant aminoalcohols as their fluorescent 1-naphthyl isocyanate derivatives

A method for determining the enantiomers of 10 therapeutically relevant ami noalcohols using HPLC and precolumn derivatization was developed. Naphthyl isocyanate reacted with racemic aminoalcohols to form urea derivatives whic h were separated isocratically on a cellulose tris(3.5-dimethylphenylcarbam ate) coated silica gel column, and detected fluorometrically in the lower n g mL(-1) range. The effluents can also be monitored at lower sensitivity, u sing an ultraviolet detector operated at 220 nm. Copyright (C) 2001 John Wi ley & Sons, Ltd.