Induction of highly polysialylated neural cell adhesion molecule (PSA-NCAM) in postischemic gerbil hippocampus mainly dissociated with neural stem cell proliferation

We investigated a possible expression of highly polysialylated neural cell adhesion molecule (PSA-NCAM) in gerbil hippocampus after 5 min of transient global ischemia in association to the proliferation of neural stern cell l abeled with bromodeoxyuridine (BrdU). The number of PSA-NCAM positive cells increased in the granule cell layer (GCL) of dentate gyrus (DG) by 1.9 to 2.7-fold at 10 and 20 days after the reperfusion. The number of BrdU-labele d cells increased mainly in the subgranular zone of DG by 7.2 to 8.0-fold a t 5 and 10 days after the reperfusion. Immunofluorescence for PSA-NCAM and BrdU showed that the majority of DG cells were not double labeled, while on e or two cells per section were double labeled in the deepest portion of th e GCL only at IO days after the reperfusion. These results suggest differen t predominant spatial distribution and chronological change of PSA-NCAM pos itive and BrdU-labeled cells in DG after transient ischemia, (C) 2001 Elsev ier Science B.V. All rights reserved.