Measurements of the 600 MHz 1D H-1-NMR and H-H COSY spectra are possible at
the nanomole order of oligosaccharide. However, many proton peaks of oligo
saccharide are observed to overlap at near delta 2 similar to 4 ppm in the
H-1-NMR spectrum. Therefore, full assignments of the H-1-NMR spectrum of ol
igosaccharide are difficult from only the ID H-1-NMR and H-H COSY spectral
data. However an analysis of the H-1-NMR spectrum of the oligosaccharide is
possible using the following three conditions: I. The splitting pattern of
the AMX type is symmetric at the chemical shift. 2. The multiplicity of pr
otons included in overlapped peaks is from the tripler to the octet (dd-ddd
). 3. In the oligosaccharide, the conformations of composite monosaccharide
s are similar to those of the corresponding monosaccharide derivatives. The
program (FORTRAN 77) for an analysis under this condition was developed, a
nd was applied to analyses of pyridylaminated-disaccharide (Gal beta1-3GalN
Ac-PA) and disaccharide-alditol (Gal beta1-3GalNAc-ol).