THE MURINE MYELOPEROXIDASE GENE CONTAINS A BIPARTITE DISTAL ENHANCER,INCLUDING A NOVEL REGION REGULATED BY PEBP2 CBF/

The myeloperoxidase (MPO) gene is expressed specifically in immature m yeloid cells. DNA (27 kb) derived from the murine MPO gene was subclon ed into nine segments, and each examined for its ability to enhance tr anscription in 32D cl3 myeloid cells. Only one segment, besides the kn own proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of th e proximal enhancer and was also recently identified in a different ce ll system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and i s regulated by PEBP2/CBF. The PEBP2/CBF binding-site in the distal enh ancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA. Copyr ight (C) 1996 Elsevier Science Ltd