High constitutive level of NF-kappa B is crucial for viability of adenocarcinoma cells

Most of cells exhibit low nuclear level of NF-kappaB, However, in some cell lines and tissues aberrantly activated NF-kappaB is playing an important r ole in cell motility, growth control and survival, Here we describe the res ult of decrease of constitutive NF-kappaB level in different adenocarcinoma cell lines. Treatment of mouse adenocarcinoma cell line CSML-100 with both synthetic (TPCK or PDTC) or natural (I kappaB-alpha) NF-kappaB inhibitors caused apoptotic death, Low doses of TPCK were harmless for CSML100 cells b ut sensitized them to TNF-induced apoptosis. Death of CSML100 cells in the presence of high concentration TPCK was not accompanied with significant ch anges in c-myc activity but strongly correlated with rapid decrease in p53 level, Thus, mutual behavior p53 and NF-kappaB represented a unique feature of TPCK-induced apoptosis in CSML-100 adenocarcinoma cells.