INDUCTION OF CHROMOSOMAL-ABERRATIONS WITH BENZON NUCLEASE IN CHINESE-HAMSTER OVARY (CHO) CELLS

Authors
JOHANNES C OBE G
Citation
C. Johannes et G. Obe, INDUCTION OF CHROMOSOMAL-ABERRATIONS WITH BENZON NUCLEASE IN CHINESE-HAMSTER OVARY (CHO) CELLS, Mutation research. Mutation research letters, 325(2-3), 1994, pp. 113-116
Citations number
11
Categorie Soggetti
Genetics & Heredity",Toxicology
Journal title
Mutation research. Mutation research letters → ACNP
ISSN journal
01657992
Volume
325
Issue
2-3
Year of publication
1994
Pages
113 - 116
Database
ISI
SICI code
0165-7992(1994)325:2-3<113:IOCWBN>2.0.ZU;2-K
Abstract
Benzon nuclease, an endonuclease originating from Serratia marcescens, was tested for its chromosome breaking activity in Chinese hamster ov ary cells. Using a permeabilizing method with hypertonic glycerol, ben zon nuclease induced chromosomal aberrations in an S-phase independent manner. The frequencies of polycentric chromosomes were correlated wi th the dose of the enzyme and the intercellular distribution of aberra tions was overdispersed.