Retroviral vector containing human p16 gene and its inhibitory effect on Bcap-37 breast cancer cells

Objective To study the effects of the p16 gene on tumor cell growth inhibit ion and cell cycle arrest. Methods The recombinant retroviral vector pLp16SN was constructed by clonin g the human pie cDNA into the retroviral vector pLXSN. Retroviruses with or without the p16 gene were obtained by transfecting pLp16SN and pLXSN vecto rs into PA317 cells. Bcap-37 human breast cancer cells were infected with t hese retroviruses followed by selection with G418. The expression of p16 wa s detected by Northern and Western blots. Cell biological characteristics, including cell growth rate, cell cycle and tumorigenesis in nude mice were assessed. Results Both mRNA and protein expression of p16 in Bcap-37 cells transfecte d with retroviral vector containing the p16 gene were much higher than that in Bcap-37 cells transfected with empty vector or parental Bcap-37 cells. Cell overexpressing the p16 gene exhibited a slower rate of growth, a highe r percentage of cells in the G(1) phase, and smaller tumors in nude mice, c ompared with parental Bcap-37 cells and cells transfected with empty vector . Conclusion Overexpression of the p16 gene could suppress the growth of Bcap -37 breast cancer cells by arresting the cell cycle at the G(1) to S-phase.