Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene an d a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The tr eated and control samples of different development stages were observed wit h a fluorescent microscope. The transient expression of GFP gene was high i n nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed t hat the foreign genes had been transferred into the shrimps and had express ed the corresponding proteins. This work has established a transgenic metho d for penaeid shrimps, which will set base for the application of genetic e ngineering breeding into industry.